Composite

Part:BBa_K5089003

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)


MGS0156

This composite part consists of all components required for expressing the MGS0156 esterase in E.coli BL21(DE3). The characterization of the enzymatic activity of purified MGS0156 against PLA is available in the documentation for the base part BBa_K4949002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1059
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 159
    Illegal NgoMIV site found at 330
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 843


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Categories
Parameters
None